Identification of key genes associated with the progression of liver fibrosis to hepatocellular carcinoma based on iTRAQ proteomics and GEO database

Liver fibrosis (LF) often leads to cirrhosis and even hepatocellular carcinoma (HCC), but the molecular mechanism remains unclear. The aims of the present study were to identify potential biomarkers for the progression of LF to HCC and explore the associated molecular mechanisms. The isobaric tags f...

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Bibliographic Details
Published inAnnals of hepatology Vol. 27; no. 3; p. 100681
Main Authors Yan, Jiongyi, Fang, Xuewan, Feng, Yinyi, Cui, Xiaojuan, Li, Fang, Luo, Weisheng, Ma, Xiaocong, Liang, Jianqin, Feng, Jianfang
Format Journal Article
LanguageEnglish
Published Mexico Elsevier España, S.L.U 01.05.2022
Elsevier
Subjects
Aldehyde Dehydrogenase, Mitochondrial
Animals
Biomarkers
Carcinoma, Hepatocellular - pathology
GEO
Humans
iTRAQ proteomics
Liver Cirrhosis - genetics
liver fibrosis
Liver Neoplasms - pathology
Proteomics
Rats
DDA
NO
NAFLD
GEPIA
DTT
LC-MS/MS
SD
DEGs
KEGG
qPCR
HBV
HPLC
iTRAQ proteomics
NSI
H&E
qRT-PCR
GO
HCC
EDTA
ECM
GEO
liver fibrosis
FFAs
CCl4
Biomarkers
iTRAQ
HSCs
HCV
LF
DEPs
CHB
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Summary:Liver fibrosis (LF) often leads to cirrhosis and even hepatocellular carcinoma (HCC), but the molecular mechanism remains unclear. The aims of the present study were to identify potential biomarkers for the progression of LF to HCC and explore the associated molecular mechanisms. The isobaric tags for relative and absolute quantitation (iTRAQ) was used to detect changes in the protein expression profiles of liver tissues and to screen the differentially expressed proteins (DEPs). The differentially expressed genes (DEGs) of LF rats and patients were screened by Gene Expression Database (GEO). Subsequently, the clinicopathological analysis of the overlapping genes in different pathological stages in HCC patients based on GEPIA database was conducted. iTRAQ proteomic analysis revealed 689, 749 and 585 DEPs in the 6W, 8W and 12W groups, respectively. ALDH2, SLC27A5 and ASNS were not only the DEPs found in rats with LF with different stages but were also the DEGs related to the pathological stages and survival in patients with HCC. ALDH2, SLC27A5 and ASNS were the potential biomarkers associated with the progression of LF to HCC.
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ISSN:1665-2681
2659-5982
DOI:10.1016/j.aohep.2022.100681