Could polymorphisms of some hormonal receptor genes, involved in folliculogenesis help in predicting patient response to controlled ovarian stimulation?
Purpose The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in selected genes, responsible for hormonal regulation of folliculogenesis, are associated with response to controlled ovarian hyperstimulation (COH) and clinical characteristics of women enrolled in in v...
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Published in | Journal of assisted reproduction and genetics Vol. 36; no. 1; pp. 47 - 55 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.01.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Purpose
The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in selected genes, responsible for hormonal regulation of folliculogenesis, are associated with response to controlled ovarian hyperstimulation (COH) and clinical characteristics of women enrolled in in vitro fertilization (IVF) programs.
Methods
In a cross-sectional study, 60 (IVF) patients underwent COH by using gonadotropin-releasing hormone (GnRH) antagonist and recombinant follicle-stimulating hormone (rFSH) protocol. Patients were classified into three groups: poor-responders (according to Bologna criteria), normo-responders (≤ 15 oocytes), and hyper-responders (> 15 oocytes). Genotyping of SNPs
AMH
rs10407022,
AMHR
rs3741664,
FSHR
rs1394205 and rs6166, and
ESR1
rs2234693 was performed using high-resolution melting analysis (HRMA). Basal FSH (bFSH), estradiol (E2), and anti-Müllerian hormone (AMH) were measured by enzyme-linked immunosorbent assay (ELISA).
Results
Patients with GG genotype of
FSHR
rs1394205 had significantly lower AMH level (
P
= 0.016) and required higher rFSH dose per oocyte compared to women with AA or AG genotype (
P
= 0.036). We also found higher frequency of GG genotype of
FSHR
rs1394205 in poor- (76.5%) than in hyper-responders (37.5%,
P
= 0.002). Patients with AA genotype of
FSHR
rs6166 had higher level of measured bFSH compared to those with AG or GG genotypes (
P
= 0.043). Women with GG genotype of
AMHR
rs3741664 required higher rFSH dose in comparison with patients carrying genotypes AA or AG (
P
= 0.028).
Conclusions
The GG genotype at position rs1394205 is associated with poor ovarian response to COH. Patients with this genotype may require higher doses of rFSH for ovulation induction. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1058-0468 1573-7330 |
DOI: | 10.1007/s10815-018-1357-4 |