Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 11; p. 3105
• Main Authors: Siriphak, Saeranee, Chanakankun, Ravinnipa, Proungvitaya, Tanakorn, Roytrakul, Sittiruk, Tummanatsakun, Doungdean, Seubwai, Wunchana, Wongwattanakul, Molin, Proungvitaya, Siriporn
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22.05.2021; MDPI
• Subjects: Antigens; Bioinformatics; Breast cancer; CCDC25; Cholangiocarcinoma; Colorectal cancer; Confidence intervals; Growth factors; Immunohistochemistry; Kallikrein; Kinases; KLK11; Liver; Lymph nodes; Lymphatic system; Malignancy; Markers; Medical prognosis; Metastases; Metastasis; mRNA; Prognosis; prognostic marker; Protein expression; Proteins; Survival analysis; Tissues; Thailand
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26113105

Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (n = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (p < 0.001 and p = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (p < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (p = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA.