Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi

• Published in: Fisheries science Vol. 82; no. 1; pp. 155 - 169
• Main Authors: Lu, Xue, Gu, Yongming, Hou, Xiaocui, Wang, Haifang, Wang, Pengfei, Xu, Peng, Zeng, Lei, Zhou, Lei, Li, Guifeng
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.01.2016; Springer Nature B.V
• Subjects: Agricultural biotechnology; Amino acids; Animals; Biomedical and Life Sciences; Cloning; compensatory growth; complementary DNA; Experiments; fasting; Feeding; Fish; Fish & Wildlife Biology & Management; Fisheries management; Food Science; Freshwater; Freshwater & Marine Ecology; gene expression; genes; Growth hormones; Insulin; Insulin-like growth factors; Laboratories; Life Sciences; Liver; messenger RNA; muscles; Musculoskeletal system; Nutrition research; Original Article; Plasma; Siniperca chuatsi; somatotropin receptors; Studies; Teleostei; Tilapia; tissue distribution; tissues; China; Fasting; Re-feeding; Growth hormone; Growth hormone receptor
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-015-0941-0

Growth hormone receptors (GHRs) are especially expressed in the muscle and liver tissues of mandarin fish Siniperca chuatsi. cDNAs of two GHR types (scGHR1 and scGHR2: 1999 and 1963 bp, encoding 638 and 578 amino acids, respectively) have been isolated and characterized in mandarin fish. Both receptors, formed through fish-specific genome duplication, have conserved FGEFS and box1 and 2 motifs. Partial compensatory growth was noted following 10-day re-feeding after 4-week fasting. After 1-week fasting, pituitary GH and muscle scGHR1 and scGHR2 mRNA levels and plasma GH concentrations increased (p < 0.05), while hepatic scGHR1 and scGHR2 mRNA expressions reduced rapidly (p < 0.05). Upon re-feeding, muscle scGHR1 and scGHR2 mRNA expressions decreased, whereas they increased rapidly in the liver, eventually normalizing. Pituitary GH mRNA expression increased to 5.1 times higher than that in the controls 3 days after re-feeding (p < 0.01), and normalized 7 days after re-feeding. The varying expression of scGHR1 and scGHR2 in different tissues suggests their distinct functions. Further, changes in GH, scGHR1, and scGHR2 mRNA expressions suggest their important roles in the growth of mandarin fish. Our results provide comparative insights into evolutionary origins and roles of GHR genes in teleost growth.