Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa

• Published in: Communications biology Vol. 2; no. 1; p. 405
• Main Authors: Testa, Samuele, Berger, Sarah, Piccardi, Philippe, Oechslin, Frank, Resch, Grégory, Mitri, Sara
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.11.2019; Nature Publishing Group
• Subjects: 631/326/1321; 631/326/2565/855; 631/326/46; Bacteria; Biofilms; Biofilms - growth & development; Biology; Biomedical and Life Sciences; Colonies; Host Microbial Interactions - physiology; Infections; Life Sciences; Liquid culture; Microscopy, Electron, Transmission; Models, Biological; Phages; Pseudomonas aeruginosa; Pseudomonas aeruginosa - classification; Pseudomonas aeruginosa - physiology; Pseudomonas aeruginosa - virology; Pseudomonas Phages - pathogenicity; Pseudomonas Phages - ultrastructure; Species Specificity; Strains (organisms); Bacteriophages; Microbial ecology; Biofilms
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-019-0633-x

Bacterial viruses, or phage, are key members of natural microbial communities. Yet much research on bacterial-phage interactions has been conducted in liquid cultures involving single bacterial strains. Here we explored how bacterial diversity affects the success of lytic phage in structured communities. We infected a sensitive Pseudomonas aeruginosa strain PAO1 with a lytic phage Pseudomonas 352 in the presence versus absence of an insensitive P. aeruginosa strain PA14, in liquid culture versus colonies on agar. We found that both in liquid and in colonies, inter-strain competition reduced resistance evolution in the susceptible strain and decreased phage population size. However, while all sensitive bacteria died in liquid, bacteria in colonies could remain sensitive yet escape phage infection, due mainly to reduced growth in colony centers. In sum, spatial structure can protect bacteria against phage infection, while the presence of competing strains reduces the evolution of resistance to phage. Testa et al. investigates the effect of strain diversity and spatial structure on success of lytic phage infecting Pseudomonas aeruginosa biofilms. They report that living in a spatially structured population can protect bacteria against phage infection, which is mainly due to slower growth at colony centre rather than any additional protection provided by presence of insensitive strain.