Hyperin protects against cisplatin-induced liver injury in mice

• Published in: Acta cirurgica brasileira Vol. 32; no. 8; pp. 633 - 640
• Main Authors: Niu, Chengwei, Ma, Man, Han, Xiao, Wang, Zimin, Li, Hangyan
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.08.2017
• Subjects: Alanine Transaminase - metabolism; Animals; Antineoplastic Agents - adverse effects; Antioxidants - pharmacology; Antioxidants - therapeutic use; Aspartate Aminotransferases - metabolism; Catalase - analysis; Chemical and Drug Induced Liver Injury; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; Cisplatin; Cisplatin - adverse effects; Cisplatin - antagonists & inhibitors; gamma-Glutamyltransferase - metabolism; Glutathione - analysis; Glutathione Peroxidase - analysis; Glutathione Transferase - analysis; Lipid Peroxidation; Liver - drug effects; Liver - enzymology; Liver - pathology; Male; Malondialdehyde - analysis; Mice; Mice, Inbred ICR; Oxidative Stress; Oxidative Stress - drug effects; Quercetin - analogs & derivatives; Quercetin - pharmacology; Quercetin - therapeutic use; Random Allocation; Reference Values; Reproducibility of Results; Superoxide Dismutase - analysis; Treatment Outcome
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/s0102-865020170080000005

To evaluate the effect of hyperin in cisplatin-induced liver injury in mice. Mice were pretreated with hyperin at doses of 25 mg/kg and 50 mg/kg, respectively, for six days, and intraperitoneal injection of cisplatin (40 mg/kg) was administrated one hour after the final intragastrication of hyperin. Twenty-four hours later, blood and liver were collected for further research. A single injection of cisplatin (40 mg/kg) for 24 h significantly increased serum alanine and aspartate aminotransferases (ALT/AST) and gamma glutamyl transferase (GGT) activities, whileas hyperin reversed cisplatin-induced such increases. Liver histopathological examination further demonstrated the protection of hyperin against cisplatin-induced liver injury. Further results showed hyperin reversed cisplatin-induced the increase in content of malondialdehyde (MDA) and the decrease in level of total antioxidant capacity (T-AOC) in liver. Moreover, hyperin increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-s transferase (GST) in cisplatin-induced liver. Hyperin inhibits cisplatin-induced hepatic oxidative stress, which contributes greatly to the amelioration of cisplatin-induced liver injury in mice.