DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters

• Published in: Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 106; no. 4; pp. 270 - 272
• Main Authors: Reinstrup, L., Jørgensen, A., Vennervald, B.J., Kristensen, T.K.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.2012; Oxford University Press
• Subjects: Animals; Biological and medical sciences; Child; DNA extraction; DNA, Helminth - isolation & purification; Egg; Female; Filtration; General aspects; Genetic variation; Humans; Male; Medical sciences; Mozambique - epidemiology; Nylon filters; Parasite Egg Count; Polymerase Chain Reaction - instrumentation; Schistosoma haematobium; Schistosoma haematobium - genetics; Schistosoma haematobium - isolation & purification; Schistosomiasis haematobia - epidemiology; Schistosomiasis haematobia - urine; Schools; Sensitivity and Specificity; Species Specificity; Students - statistics & numerical data; Urine filtration; Mozambique; Denmark, Copenhagen; Denmark; DNA extraction; Urine filtration; Schistosoma haematobium; Egg; Genetic variation; Nylon filters; Urine; Filtration; Plathelmintha; Trematoda; Filter; DNA; Helmintha; Tropical medicine; Invertebrata
• ISSN: 0035-9203; 1878-3503
• DOI: 10.1016/j.trstmh.2011.12.002

Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.