Kinetic Chemiluminescence as a Method for Oxidative Stress Evaluation in Examinations of Patients with Type 2 Diabetes Mellitus

• Published in: Bulletin of experimental biology and medicine Vol. 161; no. 1; pp. 131 - 133
• Main Authors: Proskurnina, E. V., Polimova, A. M., Sozarukova, M. M., Prudnikova, M. A., Ametov, A. S., Vladimirov, Yu. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2016; Springer; Springer Nature B.V
• Subjects: Aged; Antioxidants (Nutrients); Antioxidants - metabolism; Biomarkers - blood; Biomedical and Life Sciences; Biomedicine; Care and treatment; Cell Biology; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - diagnosis; Diabetes therapy; Experimental Methods for Clinical Practice; Female; Humans; Internal Medicine; Kinetics; Laboratory Medicine; Luminescent Measurements; Male; Medical research; Methods; Middle Aged; Oxidative Stress; Pathology; Thiobarbituric Acid Reactive Substances - metabolism; Type 2 diabetes; type 2 diabetes mellitus; blood plasma; oxidative stress; kinetic chemiluminescence
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-016-3362-x

We propose a new approach to evaluation of oxidative stress based on kinetic chemiluminescence: measurement of antioxidant and pro-oxidant activities of the plasma. The study included 50 patients with type 2 diabetes mellitus receiving peroral hypoglycemic therapy. In addition to the above parameters, the levels of TBA-reactive products, inflammation markers, clotting parameters, and biochemical values were studied. The new method provides information on oxidative stress in patients with type 2 diabetes mellitus irrespective of the clinical and laboratory values. The use of this method in complex with the clinical, laboratory, and instrumental studies allows comprehensive evaluation of patient’s status for the diagnosis and choice of therapy.