Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever

Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics, functional proteomics, and drug discovery; however, conventional biochemical sensors cannot satisfy the stringent requirements, and this has resulted in a long-standing dilemma regar...

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Published inNano research Vol. 16; no. 2; pp. 3231 - 3239
Main Authors Rao, Depeng, Yan, Tianhao, Qiao, Zihan, Wang, Yu, Peng, Yongpei, Tu, Han, Wu, Shangquan, Zhang, Qingchuan
Format Journal Article
LanguageEnglish
Published Beijing Tsinghua University Press 01.02.2023
Subjects
Antibodies
Atomic/Molecular Structure and Spectra
Biomarkers
Biomedicine
Biotechnology
Chemistry and Materials Science
Condensed Matter Physics
DNA methylation
DNA viruses
Electrons
Levers
Magnetic fields
Materials Science
Mechanical analysis
Molecular interactions
Nanotechnology
Proteins
Proteomics
Relay
Research Article
Sensitivity
Sensors
relay-type sensing mode
magneto lever
ultrasensitivity
nanomechanical sensor
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Summary:Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics, functional proteomics, and drug discovery; however, conventional biochemical sensors cannot satisfy the stringent requirements, and this has resulted in a long-standing dilemma regarding sensitivity improvement. To this end, we have developed an ultrasensitive relay-type nanomechanical sensor based on a magneto lever. By establishing the link between very weak molecular interaction and five orders of magnitude larger magnetic force, analytes at ultratrace level can produce a clearly observable mechanical response. Initially, proof-of-concept studies showed an improved detection limit up to five orders of magnitude when employing the magneto lever, as compared with direct detection using probe alone. In this study, we subsequently demonstrated that the relay-type sensing mode was universal in application ranging from micromolecule to macromolecule detection, which can be easily extended to detect enzymes, DNA, proteins, cells, viruses, bacteria, chemicals, etc. Importantly, we found that, sensitivity was no longer subject to probe affinity when the magneto lever was sufficiently high, theoretically, even reaching single-molecule resolution.
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ISSN:1998-0124
1998-0000
DOI:10.1007/s12274-022-5049-0