A Novel Method for Mouse Retinal Temperature Determination Based on ERG Photoresponses
This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature inc...
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Published in | Annals of biomedical engineering Vol. 45; no. 10; pp. 2360 - 2372 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.10.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | This study introduces a novel retinal temperature determination method based on the temperature dependent properties of photoresponses recorded by electroretinography (ERG). The kinetics and amplitudes of ERG photoresponses depend on retinal temperature. Additionally, raising retinal temperature increases the probability of long-wavelength photon absorption, which manifests as temperature dependence of photoreceptor sensitivity. In this study we extract a number of features that represent these properties from the a- and b-waves of mouse
ex vivo
ERG flash responses and construct three multivariable regression models between temperature and the selected features. The performance of these models was evaluated against a separate test dataset and for two of the models, an RMS temperature determination error of less than 0.50 °C could be reached. Our results demonstrate that the method can be successfully used for reliable retinal temperature determination
ex vivo
. The method, reflecting the temperature of distal retina, can be applied also in the estimation of retinal pigment epithelium temperature. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Associate Editor Tingrui Pan oversaw the review of this article. |
ISSN: | 0090-6964 1573-9686 |
DOI: | 10.1007/s10439-017-1872-y |