Direct observation of disulfide isomerization in a single protein

• Published in: Nature chemistry Vol. 3; no. 11; pp. 882 - 887
• Main Authors: Alegre-Cebollada, Jorge, Kosuri, Pallav, Rivas-Pardo, Jaime Andrés, Fernández, Julio M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.10.2011; Nature Publishing Group
• Subjects: 639/638/45/56; 639/638/92/469; Analytical Chemistry; Biochemistry; Chemical bonds; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cysteine - chemistry; Disulfides - chemistry; Inorganic Chemistry; Isomerism; Kinetics; Microscopy, Atomic Force; Organic Chemistry; Oxidation; Oxidation-Reduction; Photochemicals; Physical Chemistry; Polypeptides; Protein Unfolding; Proteins; Proteins - chemistry; Proteins - metabolism; Spectroscopy; Sulfhydryl Compounds - chemistry; Sulfur; New York; United States > US

Photochemical uncaging techniques use light to release active molecules from otherwise inert compounds. Here we expand this class of techniques by demonstrating the mechanical uncaging of a reactive species within a single protein. We proved this novel technique by capturing the regiospecific reacti...