Structural Characteristics, Antioxidant and Hypoglycemic Activities of Polysaccharide from Siraitia grosvenorii

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 13; p. 4192
• Main Authors: Gong, Pin, Guo, Yuxi, Chen, Xuefeng, Cui, Dandan, Wang, Mengrao, Yang, Wenjuan, Chen, Fuxin
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 29.06.2022; MDPI
• Subjects: antioxidant activity; Antioxidants; Cellulose; Chromatography; Diabetes; Hypoglycemia; hypoglycemic activity; Inflammation; Methylation; Molecular weight; Monosaccharides; Morphology; NMR; Nuclear magnetic resonance; Polysaccharides; Proteins; Siraitia grosvenorii; Siraitia grosvenorii polysaccharides; Spectrum analysis; Structural analysis; structure characterization; structure–function relationship; China; Germany
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27134192

The structural characterization, the in vitro antioxidant activity, and the hypoglycemic activity of a polysaccharide (SGP-1-1) isolated from Siraitia grosvenorii (SG) were studied in this paper. SGP-1-1, whose molecular weight is 19.037 kDa, consisted of Gal:Man:Glc in the molar ratio of 1:2.56:4.90. According to the results of methylation analysis, GC–MS, and NMR, HSQC was interpreted as a glucomannan with a backbone composed of 4)-β-D-Glcp-(1→4)-, α-D-Glcp-(1→4)-, and 4)-Manp-(1 residues. α-1,6 linked an α-D-Galp branch, and α-1,6 linked an α-D-Glcp branch. The study indirectly showed that SGP-1-1 has good in vitro hypoglycemic and antioxidant activities and that these activities may be related to the fact that the SGP-1-1’s monosaccharide composition (a higher proportion of Gal and Man) is the glycosidic-bond type (α- and β-glycosidic bonds). SGP-1-1 could be used as a potential antioxidant and hypoglycemic candidate for functional and nutritional food applications.