Adrenergic Coronary Tone during Submaximal Exercise in the Dog is Produced by Circulating Catecholamines: Evidence for Adrenergic Denervation Supersensitivity in the Myocardium but Not in Coronary Vessels

• Published in: Circulation research Vol. 58; no. 1; pp. 68 - 82
• Main Authors: Chilian, William M, Harrison, David G, Haws, Charles W, Snyder, William D, Marcus, Melvin L
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 01.01.1986; Lippincott
• Subjects: Animals; Biological and medical sciences; Blood Pressure - drug effects; Coronary Circulation - drug effects; Coronary Vessels - drug effects; Coronary Vessels - innervation; Coronary Vessels - physiology; Dogs; Epinephrine - blood; Female; Fundamental and applied biological sciences. Psychology; Heart; Heart - innervation; Heart Rate - drug effects; Isoproterenol - pharmacology; Male; Norepinephrine - blood; Norepinephrine - pharmacology; Oxygen Consumption; Physical Exertion; Propranolol - pharmacology; Receptors, Adrenergic, alpha - physiology; Receptors, Adrenergic, beta - physiology; Sympathectomy; Vascular Resistance; Vasoconstriction; Vertebrates: cardiovascular system; Heart; Physical exercise; Fissipedia; Carnivora; β-»Adrenergic receptor; Nervous control; Vasoconstriction; Catecholamine; Coronary vessel; Left ventricle; Blood flow; Vertebrata; Mammalia; α-Adrenergic receptor; Circulatory system; Hemodynamics; Dog; Sympathic nervous system
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/01.RES.58.1.68

The goal of this study was to test the hypothesis that circulating catecholamines are primarily responsible for a-adrenergic coronary vasoconstriction during submaximal exercise. The experimental series consisted of chronic studies in which a regional left ventricular sympathectomy was performed with phenol. Myocardial perfusion to the innervated and sympathectomized left ventricular regions was measured in these animals during (1) a control period, (2) treadmill exercise, (3) exercise during β-adrenergic blockade, and (4) exercise during combined α- + β-adrenergic blockade. We found no differences in myocardial perfusion between the innervated and sympathectomized regions or the transmural distribution of perfusion during any of these interventions. Thus, there is no evidence for neurogenic α-adrenergic coronary vasoconstriction. However, during exercise in the presence of α- and β-blockade, coronary resistance (mmHg±min±lOO g/ml) was significantly less in both the innervated (0.65 ± 0.07) and sympathectomized (0.68 ± 0.07) regions than during β-blockade, 0.90 ± 0.17 and 0.89 ± 0.16, respectively. This suggests that coronary α-adrenergic constriction was produced by circulating catecholamines. This concept of humorally mediated, α-adrenergic coronary vasoconstriction was strengthened by in vivo and in vitro studies that demonstrated that α-adrenergic Supersensitivity of the coronary vasculature was not present. Myocardial β-adrenergic Supersensitivity was observed in the phenol regional sympathectomy model; however, this effect was blocked by propranolol (1 mg/kg). This indicates that α-adrenergic vasoconstriction in both myocardial regions during submaximal exercise is produced by circulating catecholamines. The major conclusion of this study is that, during submaximal exercise in the canine, α-adrenergic coronary vasoconstrictor tone is predominately due to circulating catecholamines rather than direct neural effects.