The amino-terminal sequences of four major carp γ-crystallin polypeptides and their homology with frog and calf γ-crystallins

• Published in: FEBS letters Vol. 209; no. 1; pp. 107 - 110
• Main Authors: Chiou, Shyh-Horng, Chen, Shun-Wen, Lo, Tung-Bin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.1986; Elsevier
• Subjects: Amino Acid Sequence; amino acids; Analytical, structural and metabolic biochemistry; Animals; Applied sciences; Biological and medical sciences; Carps - genetics; Crystallins - genetics; Crystallins - isolation & purification; Cyprinidae - genetics; Cyprinus carpio; Exact sciences and technology; Freshwater; Fundamental and applied biological sciences. Psychology; gamma -crystallin; Holoproteins; Lens crystallin Protein sequence Sequence homology Microheterogeneity Multigene family; Lens crystallinProtein sequenceSequence homologyMicroheterogeneityMultigene family; Other proteins; Other techniques and industries; Proteins; Rana; Ranidae - genetics; Rats; Sequence Homology, Nucleic Acid; Species Specificity; Lens crystallin Protein sequence Sequence homology Microheterogeneity Multigene family; Proteins; Vertebrata; Mammalia; Frog; Pisces; N terminal-Sequence; Amphibia; Ox; Homology; Salientia; Artiodactyla; Ungulata
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)81093-6

Four major γ-crystallin subfractions have been isolated from the carp ( Cyprinus carpio) and their N-terminal sequences determined by Edman protein sequencing. Extensive homologies indicative of close relatedness in their primary structure were found in these four γ-crystallin polypeptides. Comparison of the carp N-terminal sequences with those of mammalian and amphibian γ-crystallins also showed a high degree of homology present in their N-terminal segments despite the dissimilarity of amino acid compositions of fish γ-crystallins to those of higher classes of vertebrates. The distinct yet closely-related partial sequences of carp γ-crystallins could account for the profound microheterogeneity detected in the characterization of carp crystallins, suggesting the presence of a multigene family for γ-crystallin in the lowest class of vertebrates, i.e. the fish.