Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

• Published in: The Journal of pathology Vol. 188; no. 3; pp. 289 - 293
• Main Authors: Sallinen, Satu-Leena, Sallinen, Pauli K., Kononen, Juha T., Syrjäkoski, Kirsi M., Nupponen, Nina N., Rantala, Immo S., Helén, Pauli T., Helin, Heikki J., Haapasalo, Hannu K.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.07.1999; Wiley
• Subjects: astrocytoma; Astrocytoma - metabolism; Astrocytoma - pathology; Biological and medical sciences; Biomarkers, Tumor - metabolism; brain tumours; Cell Division; cyclin D1; Cyclin D1 - genetics; Cyclin D1 - metabolism; Follow-Up Studies; Gene Expression; Humans; Medical sciences; Neoplasm Proteins - metabolism; Neurology; Prognosis; proliferation; RNA, Messenger - genetics; RNA, Neoplasm - genetics; Survival Rate; Tumors of the nervous system. Phacomatoses; Human; Immunohistochemistry; Cell proliferation; Nervous system diseases; Prognosis; Malignant tumor; Cyclin D1; Polymerase chain reaction; Pathology; Malignant glioma; Malignant astrocytoma; Central nervous system disease; Cell cycle; Molecular biology
• ISSN: 0022-3417; 1096-9896
• DOI: 10.1002/(SICI)1096-9896(199907)188:3<289::AID-PATH351>3.0.CO;2-X

An important positive regulator of the cell cycle, cyclin D1, is often amplified and overexpressed in malignancies. Cyclin D1 aberrations were analysed in grade II–IV astrocytomas by fluorescence in situ hybridization (FISH), mRNA in situ hybridization and immunohistochemistry. Proliferation activity was determined by Ki‐67MIB‐1 immunolabelling and mitotic counting. High cyclin D1 expression was observed in grade IV astrocytomas (grades II–III versus grade IV; mRNA expression: p < 0·001; immunoexpression: p = 0·013), and correlated with poor patient survival (p < 0·001, n = 46). Upregulated cyclin D1 expression was also closely associated with poor patient prognosis in grade II–III astrocytomas (p < 0·001, n = 30). Cyclin D1 gene was not found to be amplified (n = 7). Cell proliferation activity was significantly increased in tumours exhibiting high cyclin D1 mRNA levels (Ki‐67MIB‐1: p < 0·001; mitotic count: p < 0·001) and high cyclin D1 protein expression (Ki‐67MIB‐1: p = 0·002; mitotic count: p = 0·012). These results indicate that increased production of cyclin D1 is closely associated with high cell proliferation activity and aggressive behaviour in diffusely infiltrating astrocytomas. Copyright © 1999 John Wiley & Sons, Ltd.