Rationally Engineered Total Biosynthesis of a Synthetic Analogue of a Natural Quinomycin Depsipeptide in Escherichia coli

• Published in: Chembiochem : a European journal of chemical biology Vol. 10; no. 12; pp. 1965 - 1968
• Main Authors: Watanabe, Kenji, Hotta, Kinya, Praseuth, Alex P., Searcey, Mark, Wang, Clay C. C., Oguri, Hiroki, Oikawa, Hideaki
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 17.08.2009
• Subjects: antibiotics; Biological Factors - biosynthesis; Biological Factors - chemistry; Biological Factors - isolation & purification; biosynthesis; Depsipeptides - biosynthesis; Depsipeptides - chemistry; Depsipeptides - isolation & purification; Echinomycin - analogs & derivatives; Echinomycin - biosynthesis; Echinomycin - chemistry; Echinomycin - isolation & purification; Escherichia coli - chemistry; Escherichia coli - enzymology; Escherichia coli - metabolism; Genetic Engineering; Methyltransferases - genetics; Methyltransferases - metabolism; Molecular Conformation; NRPS; protein engineering

Drugs from bugs. We have successfully expanded the scope of our E. coli‐based total biosynthesis of natural products and their analogues by rationally engineering the echinomycin biosynthesis pathway for the production of a synthetic analogue of triostin A, TANDEM; this demonstrates the amenability...