Rationally Engineered Total Biosynthesis of a Synthetic Analogue of a Natural Quinomycin Depsipeptide in Escherichia coli

Drugs from bugs. We have successfully expanded the scope of our E. coli‐based total biosynthesis of natural products and their analogues by rationally engineering the echinomycin biosynthesis pathway for the production of a synthetic analogue of triostin A, TANDEM; this demonstrates the amenability...

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Published inChembiochem : a European journal of chemical biology Vol. 10; no. 12; pp. 1965 - 1968
Main Authors Watanabe, Kenji, Hotta, Kinya, Praseuth, Alex P., Searcey, Mark, Wang, Clay C. C., Oguri, Hiroki, Oikawa, Hideaki
Format Journal Article
LanguageEnglish
Published Weinheim WILEY‐VCH Verlag 17.08.2009
Subjects
antibiotics
Biological Factors - biosynthesis
Biological Factors - chemistry
Biological Factors - isolation & purification
biosynthesis
Depsipeptides - biosynthesis
Depsipeptides - chemistry
Depsipeptides - isolation & purification
Echinomycin - analogs & derivatives
Echinomycin - biosynthesis
Echinomycin - chemistry
Echinomycin - isolation & purification
Escherichia coli - chemistry
Escherichia coli - enzymology
Escherichia coli - metabolism
Genetic Engineering
Methyltransferases - genetics
Methyltransferases - metabolism
Molecular Conformation
NRPS
protein engineering
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Summary:Drugs from bugs. We have successfully expanded the scope of our E. coli‐based total biosynthesis of natural products and their analogues by rationally engineering the echinomycin biosynthesis pathway for the production of a synthetic analogue of triostin A, TANDEM; this demonstrates the amenability of the biosynthesis enzymes and E. coli towards further engineering.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
1439-7633
DOI:10.1002/cbic.200900260