Enhanced detectability in proteome studies

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 849; no. 1; pp. 91 - 104
• Main Authors: Sriyam, Supawadee, Sinchaikul, Supachok, Tantipaiboonwong, Payungsak, Tzao, Ching, Phutrakul, Suree, Chen, Shui-Tein
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.04.2007; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Biomarkers - analysis; Computational Biology - methods; Detection; Fundamental and applied biological sciences. Psychology; General pharmacology; Limitation; Medical sciences; Pharmacology. Drug treatments; Proteome; Proteome - analysis; Proteome - chemistry; Proteomic analysis; Proteomics - methods; Reproducibility of Results; Sample preparation; Proteome; Proteomic analysis; Sample preparation; Detection; Limitation; Peptides; Peptide fragment; Analysis; Proteomics; Detectability; Protein
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.10.065

The discovery of candidate biomarkers from biological materials coupled with the development of detection methods holds both incredible clinical potential as well as significant challenges. However, the proteomic techniques still provide the low dynamic range of protein detection at lower abundances. This review describes the current development of potential methods to enhance the detection and quantification in proteome studies. It also includes the bioinformatics tools that are helpfully used for data mining of protein ontology. Therefore, we believe that this review provided many proteomic approaches, which would be very potent and useful for proteome studies and for further diagnostic and therapeutic applications.