Characterization and Application of a Lytic Phage D10 against Multidrug-Resistant Salmonella

• Published in: Viruses Vol. 13; no. 8; p. 1626
• Main Authors: Li, Zhiwei, Li, Wanning, Ma, Wenjuan, Ding, Yifeng, Zhang, Yu, Yang, Qile, Wang, Jia, Wang, Xiaohong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.08.2021; MDPI
• Subjects: Adsorption; Animals; Antibiotic resistance; Antibiotics; Bacterial infections; Biological control; Biological Control Agents - isolation & purification; Burst size; Cell size; cocktail; Drug resistance; Drug Resistance, Multiple, Bacterial; Eggs; Food contamination; Food Contamination - prevention & control; Food Microbiology - methods; Food Safety - methods; Foodborne pathogens; Genome, Viral; Genomes; genomic; Genomic analysis; Glycerol; Host Specificity; Humans; Infections; Latent period; lysis; Lysogeny; Morphology; Multidrug resistance; Multidrug resistant organisms; multidrug-resistant; Pathogenicity; phage; Phages; Poultry - virology; Salmonella; Salmonella Phages - classification; Salmonella Phages - genetics; Salmonella Phages - growth & development; Salmonella Phages - pathogenicity; Salmonella typhimurium - virology; Serogroup; Siphoviridae - classification; Siphoviridae - genetics; Siphoviridae - isolation & purification; Temperature tolerance; United States > US; Salmonella; eggs; cocktail; genomic; lysis; phage; multidrug-resistant
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13081626

is a widely distributed foodborne pathogen that is a serious threat to human health. The accelerated development of drug resistance and the increased demand for natural foods invoke new biocontrol agents to limit contamination by multidrug-resistant (MDR) strains. In this study, a lytic phage named D10 was characterized at the biological and genomic levels. D10 possesses a short latent period (10 min) and a large burst size (163 PFU/cell), as well as adequate stability under a range of pH conditions and moderate thermal tolerance. D10 effectively lysed different MDR serovars and repressed their dynamic growth in the medium. Genomic analysis disclosed that D10 is a new member of the family and lacks the genes implicated in lysogeny, pathogenicity, or antibiotic resistance. A three-ingredient phage cocktail was then developed by mixing D10 with previously identified myovirus D1-2 and podovirus Pu20. The cocktail significantly reduced the count of MDR strains in liquid eggs, regardless of the temperature applied (4 and 25 °C). These results suggest that phage D10 is a promising tool to prevent food contamination by MDR .