RNA sequences that work as transcriptional activating regions

We describe a set of RNA molecules that work as transcriptional activators when tethered to DNA. These RNA activating regions were found amongst a randomized set of molecules bearing variants of a 10 nt loop attached to an RNA stem. The various RNA activating regions all bear an identical five‐ resi...

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Bibliographic Details
Published inNucleic acids research Vol. 31; no. 5; pp. 1565 - 1570
Main Authors Saha, Shamol, Ansari, Aseem Z., Jarrell, Kevin A., Ptashne, Mark
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.03.2003
Oxford Publishing Limited (England)
Subjects
Base Sequence
Binding Sites - genetics
Molecular Sequence Data
Nucleic Acid Conformation
Oligoribonucleotides - genetics
RNA - chemistry
RNA - genetics
RNA - isolation & purification
Saccharomyces cerevisiae - genetics
Transcriptional Activation - genetics
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Summary:We describe a set of RNA molecules that work as transcriptional activators when tethered to DNA. These RNA activating regions were found amongst a randomized set of molecules bearing variants of a 10 nt loop attached to an RNA stem. The various RNA activating regions all bear an identical five‐ residue sequence with an interspersed sixth residue. The result shows that although all natural activating regions characterized thus far are peptidic, this function can be served by other kinds of moieties as well.
Bibliography:istex:498A869D00C368130D26ABE2448E61C445DB971B
ark:/67375/HXZ-4H9VRS87-S
Received October 22, 2002; Revised and Accepted December 30, 2002
To whom correspondence should be addressed at Memorial Sloan Kettering Cancer Center, Box 595, 1275 York Avenue, New York, NY 10021, USA. Tel: +1 212 639 2297 or 5185; Fax: +1 212 717 3627; Email: m‐ptashne@ski.mskcc.org
 Present addresses:
 Shamol Saha, Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University School of Medicine, Boston, MA 02118, USA
 Aseem Z. Ansari, Department of Biochemistry and The Genome Center, University of Wisconsin‐Madison, Madison, WI 53706, USA
 Mark Ptashne, Molecular Biology Program, Sloan‐Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 595, New York, NY 10021, USA
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Aseem Z. Ansari, Department of Biochemistry and The Genome Center, University of Wisconsin-Madison, Madison, WI 53706, USA
Present addresses: Shamol Saha, Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University School of Medicine, Boston, MA 02118, USA
Mark Ptashne, Molecular Biology Program, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 595, New York, NY 10021, USA
To whom correspondence should be addressed at Memorial Sloan Kettering Cancer Center, Box 595, 1275 York Avenue, New York, NY 10021, USA. Tel: +1 212 639 2297 or 5185; Fax: +1 212 717 3627; Email: m-ptashne@ski.mskcc.org
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkg227