A Fosmid-Based System for the Generation of Recombinant Cercopithecine Alphaherpesvirus 2 Encoding Reporter Genes

The transmission of Macacine alphaherpesvirus 1 (McHV-1) from macaques, the natural host, to humans causes encephalitis. In contrast, human infection with Cercopithecine alphaherpesvirus 2 (CeHV-2), a closely related alphaherpesvirus from African vervet monkeys and baboons, has not been reported and...

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Published inViruses Vol. 11; no. 11; p. 1026
Main Authors Chukhno, Ekaterina, Gärtner, Sabine, Rahman Siregar, Abdul, Mehr, Alexander, Wende, Marie, Petkov, Stoyan, Götting, Jasper, Dhingra, Akshay, Schulz, Thomas, Pöhlmann, Stefan, Winkler, Michael
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 05.11.2019
MDPI
Subjects
Artificial chromosomes
Cell culture
Cell lines
cercopithecine alphaherpesvirus 2
Cloning
E coli
Encephalitis
fosmid
Gene expression
Genomes
Herpes viruses
Infections
Neurovirulence
Plasmids
recombineering
Viruses
United States > US
Cercopithecine alphaherpesvirus 2
recombineering
fosmid
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Summary:The transmission of Macacine alphaherpesvirus 1 (McHV-1) from macaques, the natural host, to humans causes encephalitis. In contrast, human infection with Cercopithecine alphaherpesvirus 2 (CeHV-2), a closely related alphaherpesvirus from African vervet monkeys and baboons, has not been reported and it is believed that CeHV-2 is apathogenic in humans. The reasons for the differential neurovirulence of McHV-1 and CeHV-2 have not been explored on a molecular level, in part due to the absence of systems for the production of recombinant viruses. Here, we report the generation of a fosmid-based system for rescue of recombinant CeHV-2. Moreover, we show that, in this system, recombineering can be used to equip CeHV-2 with reporter genes. The recombinant CeHV-2 viruses replicated with the same efficiency as uncloned, wt virus and allowed the identification of cell lines that are highly susceptible to CeHV-2 infection. Collectively, we report a system that allows rescue and genetic modification of CeHV-2 and likely other alphaherpesviruses. This system should aid future analysis of CeHV-2 biology.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v11111026