Important roles of Vilse in dendritic architecture and synaptic plasticity

• Published in: Scientific reports Vol. 7; no. 1; p. 45646
• Main Authors: Lee, Jin-Yu, Lee, Li-Jen, Fan, Chih-Chen, Chang, Ho-Ching, Shih, Hsin-An, Min, Ming-Yuan, Chang, Mau-Sun
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 03.04.2017
• Subjects: Animal memory; Animals; CA1 Region, Hippocampal - cytology; CA1 Region, Hippocampal - metabolism; CA1 Region, Hippocampal - physiology; Cdc42 protein; Dendrites - metabolism; Dendrites - physiology; Dendritic plasticity; Dendritic spines; Electrical stimuli; Embryogenesis; Female; Forebrain; Genes, Lethal - genetics; GTPase-Activating Proteins - genetics; GTPase-Activating Proteins - metabolism; Guanosine triphosphatases; Hippocampal plasticity; Hippocampus; Lethality; Male; Maze Learning - physiology; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Morphogenesis; Neuronal Plasticity - genetics; Neuronal Plasticity - physiology; Pyramidal Cells - metabolism; Pyramidal Cells - physiology; Rodents; Spatial memory; Spatial Memory - physiology; Structure-function relationships; Synaptic plasticity; Synaptic transmission; Synaptic Transmission - genetics; Synaptic Transmission - physiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep45646

Vilse/Arhgap39 is a Rho GTPase activating protein (RhoGAP) and utilizes its WW domain to regulate Rac/Cdc42-dependent morphogenesis in Drosophila and murine hippocampal neurons. However, the function of Vilse in mammalian dendrite architecture and synaptic plasticity remained unclear. In the present study, we aimed to explore the possible role of Vilse in dendritic structure and synaptic function in the brain. Homozygous knockout of Vilse resulted in premature embryonic lethality in mice. Changes in dendritic complexity and spine density were noticed in hippocampal neurons of Camk2a-Cre mediated forebrain-specific Vilse knockout (Vilse ) mice. Vilse mice displayed impaired spatial memory in water maze and Y-maze tests. Electrical stimulation in hippocampal CA1 region revealed that the synaptic transmission and plasticity were defected in Vilse mice. Collectively, our results demonstrate that Vilse is essential for embryonic development and required for spatial memory.