Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans
The objective of this study was to investigate the inhibitory effect of the crude extracts from some herbs on adherence of Streptococcus mutans ( S. mutans) ATCC 25175 and TPF-1 in vitro. Six herbs, Andrographis paniculata; Cassia alata; Chinese black tea ( Camellia sinensis); guava ( Psidium guajav...
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Published in | Journal of ethnopharmacology Vol. 92; no. 2; pp. 281 - 289 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.06.2004
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The objective of this study was to investigate the inhibitory effect of the crude extracts from some herbs on adherence of
Streptococcus mutans (
S. mutans) ATCC 25175 and TPF-1 in vitro. Six herbs,
Andrographis paniculata;
Cassia alata; Chinese black tea (
Camellia sinensis); guava (
Psidium guajava);
Harrisonia perforata and
Streblus asper, were extracted with 50 or 95% ethanol and dried. Herbal extracted solution at 0.5% concentration (w/v) was initially tested for bacterial adherence on glass surfaces. In order to identify type and effective concentration of the extracts, the extracts that showed the inhibition on glass surfaces were then tested on saliva-coated hydroxyapatite by the use of radiolabeled bacteria. To study the mechanism of action, the effect of the extracts at such concentration on glucosyltransferase and glucan-binding lectin activities were examined. It was found that all extracts, but
Streblus asper, showed significant inhibitory effect on bacterial adherence to glass surfaces. For the saliva-coated hydroxyapatite adherence assay,
Andrographis paniculata,
Cassia alata, Chinese black tea and
Harrisonia perforata could inhibit adherence of
S. mutans ATCC 25175. Chinese black tea was the strongest inhibitor followed by
Andrographis paniculata,
Cassia alata and
Harrisonia perforata, respectively. For
S. mutans TPF-1, adherence inhibition was observed from
Andrographis paniculata and
Cassia alata at similar levels. The lowest concentrations of the extracts that inhibited the adherence at least 50% were 0.5% of
Andrographis paniculata, 0.5% of
Cassia alata, 0.3% of Chinese black tea and 0.5% of
Harrisonia perforata for
S. mutans ATCC 25175. For
S. mutans TPF-1, the effective concentrations were 0.5% of
Andrographis paniculata and 0.4% of
Cassia alata. All extracts at such concentrations decreased the activity of glucosyltransferase from both strains. Only
Andrographis paniculata and
Cassia alata eliminated or decreased the activity of glucan-binding lectin from both strains. These findings suggested that
Andrographis paniculata,
Cassia alata, Chinese black tea and
Harrisonia perforata could inhibit adherence of
S. mutans ATCC 25175, while
Andrographis paniculata and
Cassia alata had an effect on
S. mutans TPF-1 in vitro at the concentrations employed in this study. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-8741 1872-7573 |
DOI: | 10.1016/j.jep.2004.03.008 |