Lung microbiome alterations in patients with anti-Jo1 antisynthetase syndrome and interstitial lung disease

• Published in: Frontiers in cellular and infection microbiology Vol. 13; p. 1321315
• Main Authors: Quintero-Puerta, Teresa, Lira-Lucio, Juan Alberto, Falfán-Valencia, Ramcés, Vega-Sánchez, Ángel E, Márquez-García, Eduardo, Mejía, Mayra, Bautista-Becerril, Brandon, Rojas-Serrano, Jorge, Ramos-Martínez, Espiridión, Buendía-Roldán, Ivette, Pérez-Rubio, Gloria
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.12.2023
• Subjects: 16S ribosomal subunit; antisynthetase syndrome; Autoantibodies; Cellular and Infection Microbiology; Humans; interstitial lung disease; Lung; Lung Diseases, Interstitial; lung microbiome; Myositis; Veillonella; 16S ribosomal subunit; Veillonella; interstitial lung disease; lung microbiome; antisynthetase syndrome
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2023.1321315

To characterize the lung microbiome in the bronchoalveolar lavage fluid (BALF) of patients with Antisynthetase Syndrome (ASSD) according to anti-Jo1 autoantibody positivity and evaluate the correlation with differential cell count and other bacterial genera in BALF. We sequenced the 16S ribosomal RNA gene in the BALF of anti-Jo1-positive (JoP, n=6) and non-Jo1-positive (NJo, n=17) patients, and the differential cell count in BALF was evaluated. The Spearman's correlation was calculated for the quantitative variables and abundance of bacterial species. The genus showed a significant decrease (p<0.01) in JoP (2.2%) in comparison to NJo (4.1%) patients. The correlation analysis showed several high (rho ≥ ± 0.7) and significant (p < 0.05) correlations. We analyzed the results obtained for the genera and other study variables. The JoP group showed that the abundance of had a high negative correlation with macrophages (rho = - 0.77) and a positive correlation with eosinophils (rho = 0.77), lymphocytes (rho = 0.77), and (rho = 1). The lung microbiome in ASSD patients differs and may affect cell composition, contributing to lung damage mechanisms. The presence of anti-Jo1 autoantibodies showed a low abundance of . This genus had a strong and positive correlation with abundance and levels of eosinophils and lymphocytes, and it showed a strong negative correlation with the percentage of macrophages.