Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2

• Published in: Journal of veterinary diagnostic investigation Vol. 24; no. 6; pp. 1151 - 1157
• Main Authors: Jin, Qianyue, Yang, Jifei, Lu, Qingxia, Guo, Junqing, Deng, Ruiguang, Wang, Yinbiao, Wang, Shihong, Wang, Shufen, Chen, Wen, Zhi, Yubao, Wang, Li, Yang, Suzhen, Zhang, Gaiping
• Format: Journal Article
• Language: English
• Published: United States 01.11.2012
• Subjects: Animals; antibodies; Antibodies, Viral - blood; antigens; antiserum; Capsid Proteins - genetics; Capsid Proteins - metabolism; Chromatography, Affinity - veterinary; Circoviridae Infections - blood; Circoviridae Infections - immunology; Circoviridae Infections - veterinary; Circovirus - classification; Circovirus - immunology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; gold; immunoaffinity chromatography; nuclear localization signals; Porcine circovirus-2; Reagent Strips; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sensitivity and Specificity; Swine; Swine Diseases - blood
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/1040638712462374

A rapid (<5 min) immunochromatographic strip using a colloidal gold-labeled antigen probe was successfully developed and applied for the detection of Porcine circovirus-2 (PCV-2) antibodies in swine. Recombinant Cap protein truncated nuclear localization signal of PCV-2, was expressed and labeled with colloidal gold. This conjugate was dispensed on a conjugate pad as the detector. Staphylococcal protein A and purified porcine anti-PCV-2 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. Sensitivity and specificity of this strip test was evaluated using PCV-2 antisera as well as other sera from pigs infected with a variety of swine viruses. For the validation of this strip test, 500 clinical swine serum samples were assessed both by the strip and a commercial enzyme-linked immunosorbent assay (ELISA) kit. The agreement between the immunochromatographic strip and ELISA kit was 94.00%. This strip possesses high sensitivity and specificity and may be useful as a candidate for rapid diagnosis of PCV-2 antibodies in the field.