An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy
The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence...
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Published in | Journal of fluorescence Vol. 25; no. 6; pp. 1801 - 1812 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.11.2015
Springer Science + Business Media |
Subjects | |
Online Access | Get full text |
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Summary: | The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modelled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as SPICE can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modelling of the entire system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response. The simplification of the analysis mathematics, and the ability to model the entire detection chain, make it possible to develop more compact instruments for remote sensing applications. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 DOE/NV/25946-2452 NST09-FMPD-PD07; AC52-06NA25946 USDOE National Nuclear Security Administration (NNSA), Office of Defense Nuclear Nonproliferation USDOE NA Office of Nonproliferation and Verification Research and Development (NA-22) |
ISSN: | 1053-0509 1573-4994 |
DOI: | 10.1007/s10895-015-1669-z |