Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients
In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition, and Methicillin-resistant (MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of t...
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Published in | Frontiers in microbiology Vol. 14; p. 1142646 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
06.04.2023
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Subjects | |
Online Access | Get full text |
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Summary: | In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition,
and Methicillin-resistant
(MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of the present study was to investigate the ability of biosynthesized silver nanoparticles from strawberries (
L.) leaf extract without a chemical catalyst to inhibit Gram-negative
and Gram-positive
isolated from COVID-19 patient's sputum. A wide range of measurements was performed on the synthesized AgNPs, including UV-vis, SEM, TEM, EDX, DLS, ζ -potential, XRD, and FTIR. UV-Visible spectral showed the absorbance at the wavelength 398 nm with an increase in the color intensity of the mixture after 8 h passed at the time of preparation confirming the high stability of the FA-AgNPs in the dark at room temperature. SEM and TEM measurements confirmed AgNPs with size ranges of ∼40-∼50 nm, whereas the DLS study confirmed their average hydrodynamic size as ∼53 nm. Furthermore, Ag NPs. EDX analysis showed the presence of the following elements: oxygen (40.46%), and silver (59.54%). Biosynthesized FA-AgNPs (ζ = -17.5 ± 3.1 mV) showed concentration-dependent antimicrobial activity for 48 h in both pathogenic strains. MTT tests showed concentration-dependent and line-specific effects of FA-AgNPs on cancer MCF-7 and normal liver WRL-68 cell cultures. According to the results, synthetic FA-AgNPs obtained through an environmentally friendly biological process are inexpensive and may inhibit the growth of bacteria isolated from COVID-19 patients. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology Reviewed by: Diaa Abd El Moneim, Arish University, Egypt; Helal F. Hetta, Assiut University, Egypt Edited by: Amr Fouda, Al-Azhar University, Egypt |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2023.1142646 |