Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients

In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition, and Methicillin-resistant (MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of t...

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Published inFrontiers in microbiology Vol. 14; p. 1142646
Main Authors Hawsawi, Nahed M, Hamad, Arshad M, Rashid, Sahar N, Alshehri, Fatma, Sharaf, Mohamed, Zakai, Shadi A, Al Yousef, Sulaiman A, Ali, Ahmed Mohamed, Abou-Elnour, Amira, Alkhudhayri, Abdulsalam, Elrefaei, Nadia Gouda, Elkelish, Amr
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 06.04.2023
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Summary:In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition, and Methicillin-resistant (MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of the present study was to investigate the ability of biosynthesized silver nanoparticles from strawberries ( L.) leaf extract without a chemical catalyst to inhibit Gram-negative and Gram-positive isolated from COVID-19 patient's sputum. A wide range of measurements was performed on the synthesized AgNPs, including UV-vis, SEM, TEM, EDX, DLS, ζ -potential, XRD, and FTIR. UV-Visible spectral showed the absorbance at the wavelength 398 nm with an increase in the color intensity of the mixture after 8 h passed at the time of preparation confirming the high stability of the FA-AgNPs in the dark at room temperature. SEM and TEM measurements confirmed AgNPs with size ranges of ∼40-∼50 nm, whereas the DLS study confirmed their average hydrodynamic size as ∼53 nm. Furthermore, Ag NPs. EDX analysis showed the presence of the following elements: oxygen (40.46%), and silver (59.54%). Biosynthesized FA-AgNPs (ζ = -17.5 ± 3.1 mV) showed concentration-dependent antimicrobial activity for 48 h in both pathogenic strains. MTT tests showed concentration-dependent and line-specific effects of FA-AgNPs on cancer MCF-7 and normal liver WRL-68 cell cultures. According to the results, synthetic FA-AgNPs obtained through an environmentally friendly biological process are inexpensive and may inhibit the growth of bacteria isolated from COVID-19 patients.
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This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology
Reviewed by: Diaa Abd El Moneim, Arish University, Egypt; Helal F. Hetta, Assiut University, Egypt
Edited by: Amr Fouda, Al-Azhar University, Egypt
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1142646