The effect of cobalt chloride administration on the synthesis of hepatic microsomal cytochrome P-450

Cobalt chloride administration to rats stimulates erythropoesis but inhibits the synthesis of hepatic microsomal P-450 and ethylmorphine N-dealkylase activity by hepatic microsomes. No effect on NADPH-cytochrome c reductase activity was observed. Phenobarbital induction of P-450 and ethylmorphine N-...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 42; no. 4; pp. 589 - 595
Main Authors Tephly, T.R., Hibbeln, P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.02.1971
Subjects
Animals
Cobalt - pharmacology
Cytochromes - biosynthesis
Depression, Chemical
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Summary:Cobalt chloride administration to rats stimulates erythropoesis but inhibits the synthesis of hepatic microsomal P-450 and ethylmorphine N-dealkylase activity by hepatic microsomes. No effect on NADPH-cytochrome c reductase activity was observed. Phenobarbital induction of P-450 and ethylmorphine N-demethylation are prevented by simultaneous administration of cobalt chloride but phenobarbital induction of NADPH-cytochrome c reductase activity is not affected. Hexobarbital oxidation by the perfused rat liver is also inhibited by prior administration of cobalt chloride. Since cobalt treatment of rats has no effect on hepatic protein synthesis over the course of these experiments, cobalt administration may be valuable as a tool for studying the role(s) of P-450 in various biological reactions.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(71)90528-6