Comparative genomics analysis of endangered wild Egyptian Moringa peregrina (Forssk.) Fiori plastome, with implications for the evolution of Brassicales order

• Published in: Frontiers in genetics Vol. 14; p. 1131644
• Main Authors: AbdAlla, Heba A M, Wanga, Vincent Okelo, Mkala, Elijah Mbandi, Amenu, Sara Getachew, Amar, Mohamed Hamdy, Chen, Lingyun, Wang, Qing-Feng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13.03.2023
• Subjects: comparative genomics; endangered; estimation time; Genetics; phylogeny; plastome; wild egyptian moringa peregrina; endangered; phylogeny; estimation time; plastome; comparative genomics; wild egyptian moringa peregrina
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2023.1131644

is a mono-genus belonging to the Moringaceae family, which includes 13 species. Among them, is plant species native to the Arabian Peninsula, Southern Sinai in Egypt, and the Horn of Africa, and comprehensive studies on its nutritional, industrial, and medicinal values have been performed. Herein, we sequenced and analyzed the initial complete chloroplast genome of . Concurrently, we analyzed the new chloroplast genome along with 25 chloroplast genomes related to species representing eight families in the Brassicales order. The results indicate that the plastome sequence of consists of 131 genes, with an average GC content of 39.23%. There is a disparity in the IR regions of the 26 species ranging from 25,804 to 31,477 bp. Plastome structural variations generated 20 hotspot regions that could be considered prospective DNA barcode locations in the Brassicales order. Tandem repeats and SSR structures are reported as significant evidence of structural variations among the 26 tested specimens. Furthermore, selective pressure analysis was performed to estimate the substitution rate within the Moringaceae family, which revealing that the and genes are under positive selective pressure. The phylogenetic analysis of the Brassicales order produced an accurate monophyletic annotation cluster of the Moringaceae and Capparaceae species, offering unambiguous identification without overlapping groups between and , which are genetically strongly associated. Divergence time estimation suggests that the two species recently diversified, 0.467 Ma. Our findings highlight the first complete plastome of the Egyptian wild-type of , which can be used for determining plastome phylogenetic relationships and systematic evolution history within studies on the Moringaceae family.