Development of hypothalamic opioid neurons: A combined immunocytochemical and [ 3H]thymidine autoradiographic study
Using a combined technique of immunocytochemistry and [ 3H]thymidine autoradiography, we have determined the “birth-date” of opioid peptide containing neurons in three hypothalamic nuclei. These include proopiomelanocortin neurons (indicated by ACTH immunoreactivity) in the arcuate nucleus, dynorphi...
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Published in | Neuropeptides (Edinburgh) Vol. 5; no. 4; pp. 477 - 480 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Ltd
01.02.1985
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Subjects | |
Online Access | Get full text |
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Summary: | Using a combined technique of immunocytochemistry and [
3H]thymidine autoradiography, we have determined the “birth-date” of opioid peptide containing neurons in three hypothalamic nuclei. These include proopiomelanocortin neurons (indicated by ACTH immunoreactivity) in the arcuate nucleus, dynorphin a neurons in the supraoptic nucleus, and [Leu]enkephalin neurons in the periventricular nucleus. Arcuate proopiomelanocortin neurons were born very early in embryonic development, with peak heavy [
3H]thymidine nuclear labelling occurring on embryonic day E12. Supraoptic dynorphin A neurons were also labelled relatively early (peak at E13). By contrast, [Leu]enkephalin neurons in the periventricular nucleus exhibited peak heavy nuclear labelling on day E14. The results indicate a differential genesis of these three opioid peptide containing neuronal groups in three different hypothalamic nuclei. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0143-4179 1532-2785 |
DOI: | 10.1016/0143-4179(85)90058-7 |