Characterization of the Mycovirome from the Plant-Pathogenic Fungus Cercospora beticola

• Published in: Viruses Vol. 13; no. 10; p. 1915
• Main Authors: Li, Yingxi, Zhou, Mengke, Yang, Yizhou, Liu, Qi, Zhang, Zongying, Han, Chenggui, Wang, Ying
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.09.2021; MDPI
• Subjects: Amino Acid Sequence; Beta vulgaris - microbiology; biocontrol; Biodiversity; Biological control; Cercospora; Cercospora - virology; Cercospora beticola; China; Disease; diversity; Foliar diseases; Fungal Viruses - classification; Fungal Viruses - genetics; Fungi; Fungicides; Genomes; High-Throughput Nucleotide Sequencing; high-throughput sequencing; Leafspot; Morphology; mycovirome; Next-generation sequencing; Pathogens; Pesticides; Phenols; Phylogeny; Plant Diseases - microbiology; Plant Diseases - virology; Plants - microbiology; Sequence analysis; Virulence; Viruses; China; Beijing China; Heilongjiang China; Inner Mongolia China; Xinjiang Uighur Autonomous Region China; Cercospora beticola; diversity; biocontrol; high-throughput sequencing; mycovirome
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13101915

Cercospora leaf spot (CLS) caused by is a devastating foliar disease of sugar beet ( ), resulting in high yield losses worldwide. Mycoviruses are widespread fungi viruses and can be used as a potential biocontrol agent for fugal disease management. To determine the presence of mycoviruses in , high-throughput sequencing analysis was used to determine the diversity of mycoviruses in 139 isolates collected from major sugar beet production areas in China. The high-throughput sequencing reads were assembled and searched against the NCBI database using BLASTn and BLASTx. The results showed that the obtained 93 contigs were derived from eight novel mycoviruses, which were grouped into 3 distinct lineages, belonging to the families and as well as some unclassified (-)ssRNA viruses in the order and . To the best of our knowledge, this is the first identification of highly diverse mycoviruses in The novel mycoviruses explored in this study will provide new viral materials to biocontrol Cercospora diseases. Future studies of these mycoviruses will aim to assess the roles of each mycovirus in biological function of in the future.