Circulating concentrations of calcitonin gene-related peptide (CGRP) in normal man determined with a new, highly sensitive radioimmunoassay

• Published in: Peptides (New York, N.Y. : 1980) Vol. 12; no. 2; pp. 365 - 369
• Main Author: Schifter, S.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.1991; Elsevier Science
• Subjects: Adolescent; Adult; Aged; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Calcitonin gene-related peptide; Calcitonin Gene-Related Peptide - blood; Calcitonin Gene-Related Peptide - standards; Drug Stability; Evaluation Studies as Topic; Female; Fundamental and applied biological sciences. Psychology; Human; Humans; Male; Middle Aged; Proteins; Radioimmunoassay; Radioimmunoassay - methods; Reference Values; Sensitivity and Specificity; Serum concentrations; Calcitonin gene-related peptide; Human; Radioimmunoassay; Serum concentrations; HPLC chromatography; Calcitonine gene related peptide; Neuropeptide; Gel permeation chromatography
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/0196-9781(91)90027-M

A highly sensitive and specific radioimmunoassay for human calcitonin gene-related peptide (CGRP) has been developed and used for determination of serum CGRP levels in 232 normal individuals, 123 females and 109 males, range of age 18 to 79 years. Mean serum concentration was 36.3 pmol/l ±6.2 (SD), and serum levels were unrelated to age and sex. The specificity of the assay was confirmed by gel chromatography, followed by HPLC analysis of extracts from normal serum using synthetic α- and β-CGRP as standards.