Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production

• Published in: World journal of microbiology & biotechnology Vol. 28; no. 1; pp. 99 - 104
• Main Authors: Zarenejad, F., Yakhchali, B., Rasooli, I.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.01.2012; Springer Nature B.V
• Subjects: Agaricus - growth & development; Agaricus - metabolism; Agaricus bisporus; Agricultural production; Analysis; Applied Microbiology; Bacteria; Base Sequence; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cloning; E coli; Environmental Engineering/Biotechnology; Field tests; Food Microbiology; Fungi; Life Sciences; Microbiology; Mushrooms; Octanols - metabolism; Original Paper; Phylogenetics; Phylogeny; Pseudomonas putida - genetics; Pseudomonas putida - isolation & purification; Pseudomonas putida - physiology; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Soil Microbiology; Studies; Mushroom growth promoting bacteria; Mushroom
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-011-0796-1

Mushrooms such as Agaricus bisporus, are cultivated for food worldwide. Fruit body initiation in Agaricus bisporus is a phase change from the vegetative to the reproductive stage which depends on the presence of a casing layer with particular physical, chemical and microbiological properties. The phase change is achieved practically by environmental manipulation and the presence of naturally occurring bacteria such as Pseuodomonas putida . In this study, 274 individual bacterial isolates were collected by screening the casing layer of 14 edible mushroom farms. The isolates were analysed with respect to biochemical properties, organic and inorganic phosphate solubilization, production of siderophore and growth in the presence of volatile compound of 1-octen-3-ol. It was found that approximately 97% of the strains were able to grow in the presence of 1-octen-3-ol and 36% were able to solubilize phosphorus. Among the isolates, 23 strains were selected as potent mushroom growth promoting bacteria (MGPB) for inoculation of the casing layer. Field experiments using these strains showed various promoting effects on production of mushroom. Finally, 2 strains (strains Bt4 and Ps7) showing the highest increase in A. bisporus production, were characterized as Pseuodomonas putida by molecular methods and identified as the best suited growth promoting inoculants for application in production farms for increasing the mushroom yield.