Intraspecific genetic diversity of the fish-infecting microsporidian parasite Pseudokabatana alburnus (Microsporidia)

• Published in: Frontiers in microbiology Vol. 14; p. 1129136
• Main Authors: Weng, Meiqi, Zhang, Xintong, Xin, Zhaozhe, Xue, Sijia, Zhang, Qianqian, Li, Aihua, Zhang, Jinyong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 09.03.2023
• Subjects: genetic recombination; genetic variation; Microbiology; microsporidia; Rpb1; sexual reproduction; sexual reproduction; genetic variation; genetic recombination; Rpb1; microsporidia
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1129136

is a xenoma-forming fish microsporidium, firstly described from the liver of the from Poyang Lake in China. In the present study, was firstly reported from the ovary of 6 other East Asian minnows, including , , , , , and . Genetic analysis revealed high sequence diversity in the ribosomal internal transcribed spacer region (ITS) and the largest subunit of RNA polymerase II (Rpb1) loci of isolated from different hosts and locations. The variation of Rpb1 mainly occurred in the 1,477-1737 bp regions. The presence of a wide variety of Rpb1 haplotypes within a single fish host, together with evidence of genetic recombination suggested that may have the intergenomic variation and sexual reproduction might be present in other hosts (possibly freshwater shrimp). Phylogenetic analysis and population genetic analysis showed that there was no geographical population divergence for . Homogeneity and high variability of ITS sequences indicates that ITS may be a suitable molecular marker to distinguish different isolates. Our data confirm the broad geographical distribution and host range of in the middle and lower reaches of the Yangtze River. Additionally, we emendated the genus to exclude the infection site, liver as one of the taxonomic criteria, and proposed that fish ovary was be the general infection site of .