The effect of ticlopidine administration to humans on the binding of adenosine diphosphate to blood platelets

• Published in: Thrombosis research Vol. 17; no. 1; pp. 19 - 27
• Main Authors: Lips, Joost P.M., Sixma, Jan J., Schiphorst, Marion E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 1980
• Subjects: Adenosine Diphosphate - metabolism; Adenosine Diphosphate - pharmacology; ADP-receptors; Binding Sites; Blood Coagulation Tests; Blood Platelets - metabolism; Collagen - pharmacology; Epinephrine - pharmacology; Human platelets; Humans; Male; Platelet Aggregation; platelet aggregation inhibitors; Prostaglandins E - pharmacology; Pyridines - administration & dosage; Thiophenes - administration & dosage; Ticlopidine; Human platelets; platelet aggregation inhibitors; ADP-receptors
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/0049-3848(80)90290-X

Administration of Ticlopidine to human volunteers resulted in a prolonged bleeding time and decreased or absent aggregation of platelets with collagen and epinephrine. Adenosine diphosphate (ADP) induced platelet aggregation was initiated by a normal shape change, but the rate of the first wave of aggregation had decreased. The second wave of aggregation was absent. ADP-binding to platelets of volunteers, consisted of two classes of binding sites:one with high affinity and one with low affinity, giving a curvilinear and a linear part in a concentration dependency curve. After Ticlopidine, the low affinity part of the curve had disappeared. Evidence will be presented that this is a specific Ticlopidine effect.