A combination DNA vaccine encoding nucleoside hydrolase 36 and glycoproteine 63 protects female but not male hamsters against Leishmania mexicana

• Published in: Parasite (Paris) Vol. 16; no. 3; pp. 227 - 230
• Main Authors: Chalé-balboa, W.G., Mut-Martin, M., Ramirez-Sierra, M.J., Garcia-Miss, M.R., Dumonteil, E.
• Format: Journal Article
• Language: English
• Published: Paris EDP Sciences 01.09.2009; Princeps
• Subjects: Animals; Biological and medical sciences; Cricetinae - immunology; Cricetinae - parasitology; Female; Fundamental and applied biological sciences. Psychology; Glycoproteins - immunology; hamster; Immunization - methods; Immunization - veterinary; Leishmania donovani; Leishmania mexicana; Leishmania mexicana - drug effects; Leishmania mexicana - enzymology; leishmaniasis; Leishmaniasis, Cutaneous - immunology; Leishmaniasis, Cutaneous - veterinary; leishmaniose; Male; Mice; N-Glycosyl Hydrolases - immunology; Protozoa; Protozoan Proteins - immunology; Sex Characteristics; vaccin; vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, DNA - therapeutic use; Kinetoplastida; Immunoprotection; Protozoa; Protozoal disease; Rodentia; Sex; Parasitosis; Leishmaniasis; Genetic vaccine; Leishmania mexicana; Infection; Vertebrata; Mammalia; DNA; Hamster
• ISSN: 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2009163227

Leishmaniasis is a group of diseases caused by protozoan parasites of the Leishmania genus. Previous studies have shown that a DNA vaccine encoding Leishmania donovani antigen nucleoside hydrolase 36 and L. mexicana glycoprotein 63 is protective in mice. We investigated here the efficacy of this DNA vaccine to induce protection in golden hamsters. Male hamsters were more susceptible to infection by Leishmania mexicana than females. Following immunization with two doses of the DNA vaccine, only females resulted protected while males developed normal lesions. La leishmaniose est un groupe de maladies causé par des parasites protozoaires du genre Leishmania. Des études antérieures ont montré qu’un vaccin d’ADN codant pour les antigènes hydrolase de nucleoside 36 de Leishmania donovani et la glycoprotéine 63 de L. mexicana est protecteur chez la souris. Nous avons étudié ici l’efficacité de ce vaccin pour induire une protection chez le hamster. Les hamsters mâles ont montré une plus grande susceptibilité à l’infection que les femelles. Après la vaccination avec deux doses de vaccin d’ADN, seules les hamsters femelles furent protégées, alors que les mâles développèrent des lésions de taille normale.