Bio-priming of banana tissue culture plantlets with endophytic Bacillus velezensis EB1 to improve Fusarium wilt resistance

• Published in: Frontiers in microbiology Vol. 14; p. 1146331
• Main Authors: Xiang, Dandan, Yang, Xiaofang, Liu, Bojing, Chu, Yuanqi, Liu, Siwen, Li, Chunyu
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 16.03.2023
• Subjects: Bacillus; banana tissue culture plantlets; biological control; endophyte; Fusarium wilt of banana; Microbiology; biological control; Fusarium wilt of banana; banana tissue culture plantlets; endophyte; Bacillus
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1146331

Tissue culture techniques have been routinely used for banana propagation and offered rapid production of planting materials with favorable genotypes and free of pathogenic microorganisms in the banana industry. Meanwhile, extensive scientific work suggests that micropropagated plantlets are more susceptible to f. sp. ( ), the deadly strain that causes Fusarium wilt of bananas than conventional planting material due to the loss of indigenous endophytes. In this study, an endophytic bacterium EB1 was isolated and characterized. EB1 shows remarkable antagonistic activity against with an inhibition rate of 75.43% and induces significant morphological and ultrastructural changes and alterations in the hyphae of . Colony-forming unit (c.f.u.) counting and scanning electron microscopy (SEM) revealed that EB1 could colonize both the surface and inner tissues of banana tissue culture plantlets. Banana tissue culture plantlets of late rooting stage bioprimed with EB1 could efficiently ward off the invasive of . The bio-priming effect could maintain in the acclimatized banana plants and significantly decrease the disease severity of Fusarium wilt and induce strong disease resistance by manipulating plant defense signaling pathways in a pot experiment. Our results provide the adaptability and potential of native endophyte EB1 in protecting plants from pathogens and infer that banana tissue culture plantlets bio-priming with endophytic microbiota could be a promising biological solution in the fight against the Fusarium wilt of banana.