Bio-priming of banana tissue culture plantlets with endophytic Bacillus velezensis EB1 to improve Fusarium wilt resistance

Tissue culture techniques have been routinely used for banana propagation and offered rapid production of planting materials with favorable genotypes and free of pathogenic microorganisms in the banana industry. Meanwhile, extensive scientific work suggests that micropropagated plantlets are more su...

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Published inFrontiers in microbiology Vol. 14; p. 1146331
Main Authors Xiang, Dandan, Yang, Xiaofang, Liu, Bojing, Chu, Yuanqi, Liu, Siwen, Li, Chunyu
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 16.03.2023
Subjects
Bacillus
banana tissue culture plantlets
biological control
endophyte
Fusarium wilt of banana
Microbiology
biological control
Fusarium wilt of banana
banana tissue culture plantlets
endophyte
Bacillus
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Summary:Tissue culture techniques have been routinely used for banana propagation and offered rapid production of planting materials with favorable genotypes and free of pathogenic microorganisms in the banana industry. Meanwhile, extensive scientific work suggests that micropropagated plantlets are more susceptible to f. sp. ( ), the deadly strain that causes Fusarium wilt of bananas than conventional planting material due to the loss of indigenous endophytes. In this study, an endophytic bacterium EB1 was isolated and characterized. EB1 shows remarkable antagonistic activity against with an inhibition rate of 75.43% and induces significant morphological and ultrastructural changes and alterations in the hyphae of . Colony-forming unit (c.f.u.) counting and scanning electron microscopy (SEM) revealed that EB1 could colonize both the surface and inner tissues of banana tissue culture plantlets. Banana tissue culture plantlets of late rooting stage bioprimed with EB1 could efficiently ward off the invasive of . The bio-priming effect could maintain in the acclimatized banana plants and significantly decrease the disease severity of Fusarium wilt and induce strong disease resistance by manipulating plant defense signaling pathways in a pot experiment. Our results provide the adaptability and potential of native endophyte EB1 in protecting plants from pathogens and infer that banana tissue culture plantlets bio-priming with endophytic microbiota could be a promising biological solution in the fight against the Fusarium wilt of banana.
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Reviewed by: Lisong Ma, Hebei Agricultural University, China; Wei Wang, Chinese Academy of Tropical Agricultural Sciences, China
Edited by: Yunfeng Li, South China Agricultural University, China
This article was submitted to Microbe and Virus Interactions with Plants, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1146331