Differentiation of Corynebacterium amycolatum, C. minutissimum, C. striatum and related species by pyrolysis-gas-liquid chromatography with atomic emission detection

• Published in: Research in microbiology Vol. 153; no. 5; pp. 307 - 311
• Main Authors: Voisin, Sébastien, Deruaz, Daniel, Freney, Jean, Renaud, François N.R.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier SAS 01.06.2002; Elsevier
• Subjects: Atomic emission detection; Bacterial Typing Techniques - methods; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Biological treatment of gaseous effluents; Biotechnology; Chromatography, Gas; Corynebacterium - chemistry; Corynebacterium - classification; Corynebacterium amycolatum; Corynebacterium freneyi; Corynebacterium minutissimum; Corynebacterium striatum; Corynebacterium xerosis; Environment and pollution; Fundamental and applied biological sciences. Psychology; Gas liquid chromatography; Humans; Identification; Industrial applications and implications. Economical aspects; Microbiology; Morphology, structure, chemical composition; Phylogeny; Pyrolysis; Pyrolysis; Atomic emission detection; Corynebacterium amycolatum; Corynebacterium minutissimum; Identification; Gas liquid chromatography; Corynebacterium xerosis; Corynebacterium freneyi; Corynebacterium striatum; Gas chromatography; Corynebacterium; Phylogenetic tree; Atomic emission spectrometry; Bacteria; Actinomycetes; Corynebacteriaceae; Chemical composition; Method; Detection
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/S0923-2508(02)01324-4

We report here the application of pyrolysis-gas chromatography followed by atomic emission detection (AED) for the characterisation of Corynebacterium amycolatum and related species (i.e., C. striatum, C. minutissimum, C. xerosis and the recently described C. freneyi). This phenotypic method, which analyses the whole chemical composition of bacteria, clearly separates C. amycolatum from other species. Moreover, this C. amycolatum group is subdivided into two distinct subgroups. We cannot differentiate the C. minutissimum strains from those of C. striatum. On the other hand, C. freneyi and C. xerosis are clearly distinct from the other species.