State-of-the-art in fast liquid chromatography–mass spectrometry for bio-analytical applications

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 927; pp. 3 - 21
• Main Authors: Núñez, Oscar, Gallart-Ayala, Héctor, Martins, Claudia P.B., Lucci, Paolo, Busquets, Rosa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.05.2013
• Subjects: Animals; Bio-analysis; Biomarkers - analysis; Chromatography; Chromatography, Liquid - methods; Cleaning; Column packings; cost effectiveness; Extraction; high performance liquid chromatography; High resolution mass spectrometry; Humans; Liquid chromatography; Mass spectrometry; MIP and RAM technology; Molecular Imprinting; On-line SPE; particle size; polymers; Separation; Solid Phase Extraction; State of the art; Tandem Mass Spectrometry - methods; turbulent flow; Turbulent flow chromatography; UHPLC column technology; ultra-performance liquid chromatography; On-line SPE; FDA; CBA; LLE; Turbulent flow chromatography; SBSE; Bio-analysis; MSn; ISRP; APCI; SCID; IT-TOF; ATCA; NNAL; SRM; ADS; PRS; TFC; HRMS; SPME; PPT; HETP; ESI; UHPLC; LSD; HILIC; MIP and RAM technology; AIF; RSD; MS; High resolution mass spectrometry; HSS; MISBSE; LOQ; UHPLC column technology; NIP; MIP; PE; APPI; FWHM; API; RAM
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2012.12.031

► State-of-the-art in fast LC–MS bioanalytical applications is presented. ► Main problems in the sample treatment and LC–MS analysis are discussed. ► On-line SPE, MIPs, RAM and TFC are addressed with relevant applications. ► Novel applications of sub-2μm and porous shell columns are discussed. ► Novelties in HRMS for bioanalysis are presented. There is an increasing need of new bio-analytical methodologies with enough sensitivity, robustness and resolution to cope with the analysis of a large number of analytes in complex matrices in short analysis time. For this purpose, all steps included in any bio-analytical method (sampling, extraction, clean-up, chromatographic analysis and detection) must be taken into account to achieve good and reliable results with cost-effective methodologies. The purpose of this review is to describe the state-of-the-art of the most employed technologies in the period 2009–2012 to achieve fast analysis with liquid chromatography coupled to mass spectrometry (LC–MS) methodologies for bio-analytical applications. Current trends in fast liquid chromatography involve the use of several column technologies and this review will focus on the two most frequently applied: sub-2μm particle size packed columns to achieve ultra high pressure liquid chromatography (UHPLC) separations and porous-shell particle packed columns to attain high efficiency separations with reduced column back-pressures. Additionally, recent automated sample extraction and clean-up methodologies to reduce sample manipulation, variability and total analysis time in bio-analytical applications such as on-line solid phase extraction coupled to HPLC or UHPLC methods, or the use of other approaches such as molecularly imprinted polymers, restricted access materials, and turbulent flow chromatography will also be addressed. The use of mass spectrometry and high or even ultra-high resolution mass spectrometry to reduce sample manipulation and to solve ion suppression or ion enhancement and matrix effects will also be presented. The advantages and drawbacks of all these methodologies for fast and sensitive analysis of biological samples are going to be discussed by means of relevant applications.