Reduction of Enzyme Dosage by Oxygen Delignification and Mechanical Refining for Enzymatic Hydrolysis of Green Liquor-Pretreated Hardwood

• Published in: Applied biochemistry and biotechnology Vol. 165; no. 3-4; pp. 832 - 844
• Main Authors: Koo, Bon-Wook, Treasure, Trevor H., Jameel, Hasan, Phillips, Richard B., Chang, Hou-min, Park, Sunkyu
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.10.2011; Springer; Springer Nature B.V
• Subjects: beta-Glucosidase - metabolism; Biochemistry; Biofuels; Biological and medical sciences; Biomass; Bioreactors; Biotechnology; Carbonates - chemistry; Cellulase - metabolism; Chemistry; Chemistry and Materials Science; Endo-1,4-beta Xylanases - metabolism; Enzymes; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Hydrology; Hydrolysis; Industrial Microbiology - methods; Lignin - metabolism; Liquor; Monosaccharides - biosynthesis; Oxygen; Porosity; Sugar; Sulfides - chemistry; Temperature; Wood; Wood - metabolism; Reduction of enzyme dosage; Green liquor pretreatment; Oxygen delignification; Enzyme accessibility; Post-treatment; Mechanical refining; Oxygen; Accessibility; Enzyme; Hardwood; Delignification; Hydrolysis; Reduction; Treatment; Pretreatment
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-011-9301-4

In this study, a strategy to reduce enzyme dosage is evaluated by applying two post-treatments, oxygen delignification and mechanical refining. The sugar conversion for GL12 substrates was increased from 51.5% to 77.9% with post-treatments at the enzyme dosage of 10 FPU. When the amount of enzyme was reduced to 5 FPU with post-treatments, the conversion of 71.8% was obtained, which was significant higher than the conversion without any post-treatment using 10 FPU (51.5%). This clearly demonstrates the benefit of post-treatments that allows more than 50% of enzyme reduction at the same level of enzymatic conversion. Enzyme-accessible surface area and pore volume were evaluated by Simons' staining and DSC thermoporometry methods, and strong correlations were found with the sugar conversion.