Rapid detection of Staphylococcus aureus and methicillin resistance in bone and joint infection samples: evaluation of the GeneXpert MRSA/SA SSTI assay

Abstract The GeneXpert MRSA/SA SSTI assay was compared to conventional cultures to detect Staphylococcus aureus and methicillin-resistance from 91 bone and joint infection samples. Sensitivity and specificity were 94.4% and 100%. Three false-positive results were observed, in fact providing from pat...

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Published inDiagnostic microbiology and infectious disease Vol. 78; no. 3; pp. 313 - 315
Main Authors Valour, Florent, Blanc-Pattin, Viviane, Freydière, Anne-Marie, Bouaziz, Anissa, Chanard, Emmanuel, Lustig, Sébastien, Ferry, Tristan, Laurent, Frédéric
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.2014
Elsevier
Subjects
Arthritis, Infectious - diagnosis
Arthritis, Infectious - microbiology
Bacteriological Techniques - methods
Biomechanics
Bone and joint infection
Bone Diseases - diagnosis
Bone Diseases - microbiology
Engineering Sciences
GeneXpert MRSA/SA SSTI assay
Humans
Infectious Disease
Internal Medicine
Mechanics
Methicillin-resistance
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Real time polymerase chain reaction
Staphylococcal Infections - diagnosis
Staphylococcus aureus
Staphylococcus aureus - isolation & purification
GeneXpert MRSA/SA SSTI assay
Bone and joint infection
Methicillin-resistance
Staphylococcus aureus
Real time polymerase chain reaction
METHICILLIN-RESISTANCE
OS
REAL TIME POLYMERASE CHAIN REACTION
BIOMECANIQUE
STAPHYLOCOCCUS AUREUS
GENEXPERT MRSA/SA SSTI ASSAY
BONE AND JOINT INFECTION
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Summary:Abstract The GeneXpert MRSA/SA SSTI assay was compared to conventional cultures to detect Staphylococcus aureus and methicillin-resistance from 91 bone and joint infection samples. Sensitivity and specificity were 94.4% and 100%. Three false-positive results were observed, in fact providing from patients known to be infected by S. aureus on the basis of other concomitant osteoarticular samples, which suggests that PCR was more sensitive than culture. This diagnosis accuracy may help shorten toxic and non-optimal empirical therapies such as glycopeptides in case of methicillin-susceptible strains.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2013.11.026