First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose ( Urva auropunctata )
Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase ( ) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses ( ) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial gene (~300 bp, hereafter...
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Published in | Viruses Vol. 13; no. 11; p. 2194 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
30.10.2021
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (
) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (
) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial
gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3-99.6% and 97-98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species
). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus
. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1999-4915 1999-4915 |
DOI: | 10.3390/v13112194 |