SNPbox: web-based high-throughput primer design from gene to genome

SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts t...

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Published inNucleic acids research Vol. 32; no. suppl-2; pp. W170 - W172
Main Authors Weckx, Stefan, De Rijk, Peter, Van Broeckhoven, Christine, Del-Favero, Jurgen
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.07.2004
Oxford Publishing Limited (England)
Subjects
Databases, Nucleic Acid
DNA Primers
Exons
Genome
Internet
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Software
User-Computer Interface
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Summary:SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.
Bibliography:To whom correspondence should be addressed. Tel: +32 3 820 2321; Fax: +32 3 820 2541; Email: jurgen.delfavero@ua.ac.be
Received February 12, 2004; Revised and Accepted March 9, 2004
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2004, the authors
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkh369