Developmental genetic underpinnings of a symbiosis-associated organ in the fungus-farming ambrosia beetle Euwallacea validus

• Published in: Scientific reports Vol. 13; no. 1; p. 14014
• Main Authors: Spahr, Ellie J., Wasef, Fady, Kasson, Matt T., Kijimoto, Teiya
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.08.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/136/1660; 631/181/2806; Animals; Computed tomography; Evolution; Farming; Females; Fungi; Fungus gardens; Gene expression; Genetics; Humanities and Social Sciences; Morphology; multidisciplinary; Mutualism; RNA-mediated interference; Science; Science (multidisciplinary); Soil sciences; Symbiosis; Tomography
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-40296-1

Mutualistic interactions between organisms often mediate the innovation of traits essential to maintain the relationship. Yet our understanding of these interactions has been stymied due to various hurdles in studying the genetics of non-model animals. To understand the genetic mechanisms by which such traits develop, we examined the function of genes breathless ( btl ), trachealess ( trh ), and doublesex in the development of a novel fungus-carrying organ (mycangium) that facilitates an obligate relationship between fungus-farming ambrosia beetles and specific fungal partners. Gene knockdown by RNA interference and subsequent micro-computed tomography visualization suggest btl and trh are required for initiation of mycangia and that tubulogenesis may have been co-opted for early mycangial development.