A method of relating craniofacial sections to topography in embryos

The transparent properties of the embedding agent glycol methacrylate facilitate orientation of the complex embryonic craniofacial region. This technique allows for consistent and reproducible section-to-topography orientation. We find it to be a valuable adjunct for envisioning three-dimensional re...

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Bibliographic Details
Published inStain technology Vol. 52; no. 6; p. 327
Main Authors Zingeser, M R, McNulty, W P
Format Journal Article
LanguageEnglish
Published United States 01.01.1977
Subjects
Animals
Brain - anatomy & histology
Embryo, Mammalian - anatomy & histology
Face - anatomy & histology
Haplorhini
Macaca mulatta
Microtomy - methods
Polyhydroxyethyl Methacrylate
Staining and Labeling
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Summary:The transparent properties of the embedding agent glycol methacrylate facilitate orientation of the complex embryonic craniofacial region. This technique allows for consistent and reproducible section-to-topography orientation. We find it to be a valuable adjunct for envisioning three-dimensional relationships. The contrast of external features of the embryo is enhanced when stained lightly with hematoxylin prior to embedding. The craniofacial region of the embedded embryo is removed with a fine surgical saw and reimbedded. Section-to-topography relationships are readily monitored and documented photographically. Furthermore, it is possible to "preview" sections for symmetry and other considerations of orientation by viewing the cutting face of the block under oblique illumination. A relief image of structures is then visible.
ISSN:0038-9153
DOI:10.3109/10520297709116808