High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis

• Published in: Viruses Vol. 13; no. 4; p. 595
• Main Authors: do Socorro Fôro Ramos, Endrya, Rosa, Ulisses Alves, de Oliveira Ribeiro, Geovani, Villanova, Fabiola, de Pádua Milagres, Flávio Augusto, Brustulin, Rafael, Dos Santos Morais, Vanessa, Bertanhe, Mayara, Marcatti, Roberta, Araújo, Emerson Luiz Lima, Witkin, Steven S, Delwart, Eric, Luchs, Adriana, da Costa, Antonio Charlys, Leal, Élcio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.03.2021; MDPI
• Subjects: acute gastroenteritis; Aseptic meningitis; echovirus E; enterovirus; Gastroenteritis; Genetic diversity; Genomes; Genotypes; Geographical distribution; gut virome; Heart diseases; Meningitis; Myocarditis; Next-generation sequencing; Paralysis; pediatric virome; Phylogenetics; Phylogeny; Proteins; Surveillance; Viruses; Brazil; United States > US; gut virome; enterovirus; pediatric virome; acute gastroenteritis; echovirus E
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13040595

Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.