p75NTR antibody-conjugated microspheres: an approach to guided tissue regeneration by selective recruitment of endogenous periodontal ligament cells

• Published in: Frontiers in bioengineering and biotechnology Vol. 12; p. 1338029
• Main Authors: Zou, Xuqiang, Xie, Bo, Peng, Xuelian, Lu, Mingjie, Xu, Dan, Yuan, Hongyan, Zhang, Yixin, Wang, Di, Zhao, Manzhu, Liu, Rui, Wen, Xiujie
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2024
• Subjects: antibody conjugation; Bioengineering and Biotechnology; cell recruitment; chitosan microspheres; nano-hydroxyapatite; p75NTR; periodontal ligament cells; chitosan microspheres; periodontal ligament cells; cell recruitment; nano-hydroxyapatite; p75NTR; antibody conjugation
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2024.1338029

Repairing defects in alveolar bone is essential for regenerating periodontal tissue, but it is a formidable challenge. One promising therapeutic approach involves using a strategy that specifically recruits periodontal ligament cells (PDLCs) with high regenerative potential to achieve regeneration of alveolar bone. In this study, we have created a new type of microsphere conjugated with an antibody to target p75 neurotrophin receptor (p75NTR), which is made of nano-hydroxyapatite (nHA) and chitosan (CS). The goal of this design is to attract p75NTR hPDLCs selectively and promote osteogenesis. experiments demonstrated that the antibody-conjugated microspheres attracted significantly more PDLCs compared to non-conjugated microspheres. Incorporating nHA not only enhances cell adhesion and proliferation on the surface of the microsphere but also augments its osteoinductive properties. Microspheres effectively recruited p75NTR cells at bone defect sites in SD rats, as observed through immunofluorescent staining of p75NTR antibodies. This p75NTR antibody-conjugated nHA/CS microsphere presents a promising approach for selectively recruiting cells and repairing bone defects.