Molecular Characterization and Cross-Reactivity of Feline Calicivirus Circulating in Southwestern China

• Published in: Viruses Vol. 13; no. 9; p. 1812
• Main Authors: Zhou, Long, Fu, Nengsheng, Ding, Lu, Li, Yan, Huang, Jian, Sha, Xue, Zhou, Qun, Song, Xin, Zhang, Bin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12.09.2021; MDPI
• Subjects: Amino acids; Animal shelters; Animals; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Breakpoints; Caliciviridae Infections - epidemiology; Caliciviridae Infections - immunology; Calicivirus, Feline - genetics; Calicivirus, Feline - immunology; Capsid protein; Cat Diseases - epidemiology; Cat Diseases - virology; Cats; Cell culture; China - epidemiology; Cloning; Cross Reactions - immunology; Cross-reactivity; feline calicivirus; Female; Genetic diversity; Genomes; Immunization; Mice; Mice, Inbred BALB C; molecular characterization; neutralizing antibodies; Pets; phylogenetic analysis; Phylogenetics; Phylogeny; Polymerase chain reaction; Vaccination; Vaccines; Virulence; Viruses; China; United States > US; neutralizing antibodies; phylogenetic analysis; cross-reactivity; feline calicivirus; molecular characterization
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13091812

Feline calicivirus (FCV) is an important pathogen of cats that has two genogroups (GI and GII). To investigate the prevalence and molecular characteristics of FCVs in southwestern China, 162 nasal swab samples were collected from cats in animal shelters and pet hospitals. In total, 38 of the clinical samples (23.46%) were identified as FCV positive using nested RT-PCR. Phylogenetic analyses using 10 capsid protein VP1 sequences revealed that 8 GI and 2 GII strains formed two independent clusters. Additionally, three separated FCVs that were not clustered phylogenetically (two GI and one GII strains) were successfully isolated from clinical samples and their full-length genomes were obtained. Phylogenetic and recombinant analyses of a GI FCV revealed genomic breakpoints in ORF1 and ORF2 regions with evidence for recombinant events between GI sub-genogroups, which is reported in China for the first time. Furthermore, sera obtained from mice immunized independently with the three FCV isolates and a commercial vaccine were used to evaluate the cross-reactivity of neutralizing antibodies. The three separate FCVs were neutralized by each other at a 1:19 to 1:775 titer range, whereas the triple-inactivated vaccine was at a titer of 1:16, which suggested that different genogroup/sub-genogroup FCV strains exhibit significantly different titers of neutralizing antibodies, including the commercial FCV vaccine. Thus, our study revealed the genetic diversity and complex cross-reactivity levels of FCVs in southwestern China, which provides new insights for application in vaccination strategies.