Pseudogene BMI1P1 expression as a novel predictor for acute myeloid leukemia development and prognosis

• Published in: Oncotarget Vol. 7; no. 30; pp. 47376 - 47386
• Main Authors: Zhou, Ling-Yu, Zhai, Ling-Ling, Yin, Jia-Yu, Vanessa, Minse Evola-Deniz, Zhou, Jiao, Zhang, Jing, Tang, Xi, Lin, Jiang, Qian, Jun, Deng, Zhao-Qun
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 26.07.2016
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Child; Female; Humans; Leukemia, Myeloid, Acute - etiology; Leukemia, Myeloid, Acute - genetics; Leukemia, Myeloid, Acute - mortality; Male; Middle Aged; Polycomb Repressive Complex 1 - genetics; Prognosis; Pseudogenes; Research Paper; Young Adult; BMI1P1; pseudogene; acute myeloid leukemia; tumor marker
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.10156

The BMI1P1 levels of 144 de novo AML patients and 36 healthy donors were detected by real-time quantitative PCR (RQ-PCR). BMI1P1 was significantly down-regulated in AML compared with control (P < 0.001). A receiver operating characteristic (ROC) curve revealed that BMI1P1 expression could differentiate patients with AML from control subjects (AUC = 0.895, 95% CI: 0.835-0.954, P < 0.001). The percentage of blasts in bone marrow (BM) was significantly lower in BMI1P1 high-expressed group versus low-expressed group (P = 0.008). BMI1P1 high-expressed cases had significantly higher complete remission (CR) than BMI1P1 low-expressed cases (P = 0.023). Furthermore, Kaplan-Meier demonstrated that both whole AML cohort and non-M3-AML patients with low BMI1P1 expression showed shorter leukemia free survival (LFS, P = 0.002 and P = 0.01, respectively) and overall survival (OS, P < 0.001 and P = 0.011, respectively) than those with high BMI1P1 expression. Multivariate analysis also showed that BMI1P1 over-expression was an independent favorable prognostic factor for OS in both whole and non-M3 cohort of AML patients (HR = 0.462, 95% CI = 0.243-0.879, P = 0.019 and HR = 0.483, 95% CI = 0.254-0.919, P = 0.027). To further investigate the significance of BMI1P1 expression in the follow-up of AML patients, we monitored the BMI1P1 level in 26 de novo AML patients and found that the BMI1P1 level increased significantly from the initial diagnosis to post-CR (P < 0.001). These results indicated that BMI1P1 might contribute to the diagnosis of AML and the assessment of therapeutic effect.